Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names sirtuin type 1|sirtuin 1|sirtuin (silent mating type information regulation 2, S. cerevisiae, homolog) 1|sir2-like 1|SIR2alpha|OTTHUMP00000060745|SIR2L1|RP11-57G10.3|sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae)|SIRT1 | |
Accession ID NP_036370.2 | |
Blocking Peptide EBP07400 | |
Immunogen Peptide with sequence C-EITEKPPRTQKE, from the internal region of the protein sequence according to NP_036370.2. | |
Peptide Sequence C-EITEKPPRTQKE | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human, Mouse, Rat, Dog | |
Reactive Species Mouse | |
Human Gene ID 23411 | |
Mouse Gene ID 93759 | |
Rat Gene ID 309757 | |
Product Grade  | |
ELISA Detection Limit Antibody detection limit dilution 1:64000. | |
Western Blot Approx 110kDa band observed in lysates of cell line NIH3T3 (calculated MW of 80.3kDa according to Mouse NP_062786.1). Preliminary testing also showed a band at approx 120xkDa in Fetal Mouse Brain lysate. This molecular weight is routinely observed by other sources. Recommended concentration: 1-3µg/ml. Primary incubation 1 hour at room temperature. | |
Application Type Pep-ELISA, WB |
Goat Anti-SIRT1 Antibody
$423.00
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Selected References [{"pmid": 22087287, "intro": "This antibody (previous batch) has been successfully used in Western blot on Dog:", "title": "Involvement of FOXO transcription factors, TRAIL-FasL/Fas, and sirtuin proteins family in canine coronavirus type II-induced apoptosis.", "author": "Marfè G, Tafani M, Fiorito F, Pagnini U, Iovane G, De Martino L.", "journal": "PLoS One. 2011;6(11)."}] |