Goat Anti-Sirtuin 1 / SIRT1 (aa577-590) Antibody

$423.00

Category: Primary Antibodies
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SKU: EB10826
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
sirtuin type 1| sir2-like 1| hSIR2| SIR2alpha| SIR2-like protein 1| OTTHUMP00000198112| OTTHUMP00000198111| NAD-dependent deacetylase sirtuin-1| SIR2L1| sirtuin 1|SIRT1
Accession ID
NP_036370.2; NP_001135970.1
Blocking Peptide
EBP10826
Immunogen
Peptide with sequence C-EKPQEVQTSRNVES, from the internal region of the protein sequence according to NP_036370.2; NP_001135970.1.
Product Comments
This antibody is expected to recognize both reported isoforms (NP_036370.2; NP_001135970.1).
Peptide Sequence
C-EKPQEVQTSRNVES
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse
Reactive Species
Human
Human Gene ID
23411
Mouse Gene ID
93759
Product Grade
IHC Results
In paraffin embedded Human Skin shows perinuclear staining in select keratinocytes. Recommended concentration, 3-5µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:64000.
Western Blot
Approx 120kDa band observed in lysates of cell line Jurkat (calculated MW of 81.7kDa according to NP_036370.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Cheng et al, Proc Natl Acad Sci U S A. 2003 Sep 16;100(19):10794-9.; PMID: 12960381). Recommended concentration: 0.05-0.2µg/ml.
Application Type
Pep-ELISA, WB, IHC

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