Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names sodium potassium chloride cotransporter 2|Na-K-2Cl cotransporter|NKCC2A variant A|NKCC2|MGC48843|BSC1|solute carrier family 12 (sodium/potassium/chloride transporters), member 1|SLC12A1 | |
Usage Summary IF/ICC: This antibody has been successfully used in IF on Mouse: Alshahrani S et al. (2015) PMID: 26400961. This antibody has been successfully used in ICC on Pig: Andreas Brännström et al. (2022) PMID: 36471055. | |
Accession ID NP_000329.2 | |
Blocking Peptide EBP09143 | |
Immunogen Peptide with sequence C-PHYEETSFGDEAQKR, from the internal region of the protein sequence according to NP_000329.2. | |
Peptide Sequence C-PHYEETSFGDEAQKR | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human | |
Reactive Species Human, Mouse, Pig | |
Human Gene ID 6557 | |
Product Grade  | |
IHC Results In paraffin embedded Human Kidney shows strong membrane staining in some CT cells. Recommended concentration, 3-6µg/ml. | |
ELISA Detection Limit Antibody detection limit dilution 1:32000. | |
Western Blot This anitbody has been successfully used in WB on Mouse: Alshahrani S et al. (2015) PMID: 26400961. | |
Application Type Pep-ELISA, WB, IHC, IF, ICC |
Goat Anti-SLC12A1 / NKCC Antibody
$464.00
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Selected References [{"pmid": 36471055, "intro": "This antibody has been successfully used in ICC on Pig:", "title": "Intermittent thoracic resuscitative endovascular balloon occlusion of the aorta improves renal function compared to 60 min continuous application after porcine class III hemorrhage.", "author": "Andreas Brännström, Michael Hultström, Jenny Gustavsson, Zabih Aurfan & Mattias Günther", "journal": "Eur J Trauma Emerg Surg (2022). https://doi.org/10.1007/s00068-022-02189-2"}, {"pmid": 26400961, "intro": "This antibody has been successfully used in WB and IF in Mouse:", "title": "Increased Slc12a1 expression in ?-cells and improved glucose disposal in Slc12a2 heterozygous mice.", "author": "Alshahrani S, Almutairi MM, Kursan S, Dias-Junior E, Almiahuob MM, Aguilar-Bryan L, Di Fulvio M.", "journal": "J Endocrinol. 2015 Dec;227(3):153-65."}] |