Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names epididymis secretory protein Li 44|hSod1|HEL-S-44|superoxide dismutase, cystolic|superoxide dismutase 1, soluble|indophenoloxidase A|SOD, soluble|Cu/Zn superoxide dismutase|SOD|IPOA|ALS1|ALS|HGNC:11179|superoxide dismutase 1, soluble (amyotrophic lateral sclerosis 1 (adult))|SOD1 | |
Usage Summary Immunofluorescence: Strong expression of the protein seen in the nucleus and cytoplasm of U2OS cells and the cytoplasm of A431 cells. Recommended concentration: 10µg/ml.
Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371. | |
Accession ID NP_000445.1 | |
Blocking Peptide EBP07208 | |
Immunogen Peptide with sequence C-SRKHGGPKDEERH, from the internal region of the protein sequence according to NP_000445.1. | |
Peptide Sequence C-SRKHGGPKDEERH | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human, Mouse, Rat, Dog | |
Reactive Species Human, Mouse | |
Human Gene ID 6647 | |
Mouse Gene ID 20655 | |
Rat Gene ID 24786 | |
Product Grade  | |
ELISA Detection Limit Antibody detection limit dilution 1:8000. | |
Western Blot Approx 18kDa band observed in Mouse Brain and Rat Spinal Cord lysates, and in lysates of cell line NIH3T3. Approx 20kDa observed in lysates of cell lines HEK293, HepG2 and MCF7 (calculated MW of 15.9kDa according to Human NP_000445.1, Mouse NP_035564.1 and Rat NP_058746.1). Recommended concentration: 0.01-0.03µg/ml. Primary incubation 1 hour at room temperature. This antibody has been successfully used in Western blot on Mouse: Frazziano G et al. (2014) PMID: 24213612. | |
Application Type Pep-ELISA, WB, IF |
Goat Anti-SOD1 Antibody
$423.00
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Selected References [{"pmid": 24213612, "intro": "This antibody has been successfully used in Western blot on Mouse:", "title": "Nox-derived ROS are acutely activated in pressure overload pulmonary hypertension: indications for a seminal role for mitochondrial Nox4.", "author": "Frazziano G, Al Ghouleh I, Baust J, Shiva S, Champion HC, Pagano PJ.", "journal": "Am J Physiol Heart Circ Physiol. 2014 Jan 15;306(2):H197-205."}, {"pmid": 30377371, "intro": "This antibody has been successfully used in the following paper:", "title": "A high-throughput pipeline for validation of antibodies", "author": "Krzysztof Sikorski, Adi Mehta, Marit Inngjerdingen, Flourina Thakor, Simon Kling, Tomas Kalina, Tuula A. Nyman, Maria Ekman Stensland, Wei Zhou, Gustavo A. De Souza, Lars Holden, Jan Stuchly, Markus Templin and Fridtjof Lund-Johansen", "journal": "Nat Methods. 2018 Nov;15(11):909-912"}] |