Goat Anti-SOX11 (aa309-323) Antibody

$423.00

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SKU: EB11231
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
SOX11| transcription factor SOX-11| SRY-related HMG-box gene 11| SRY (sex-determining region Y)-box 11| SRY (sex determining region Y)-box 11
Accession ID
NP_003099.1
Blocking Peptide
EBP11231
Immunogen
Peptide with sequence C-SRLYYSFKNITKQHP, from the internal region of the protein sequence according to NP_003099.1.
Peptide Sequence
C-SRLYYSFKNITKQHP
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Pig, Cow
Reactive Species
Human
Human Gene ID
6664
Mouse Gene ID
20666
Rat Gene ID
84046
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:2000.
Western Blot
Approx 65kDa band observed in lysates of cell lines Daudi, Jurkat and MOLT4 (calculated MW of 46.7kDa according to NP_003099.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Dictor et al, Haematologica. 2009 Nov;94(11):1563-8. PMID: 19880779). Recommended concentration: 0.3-1µg/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on NIH3T3, Mouse and Rat Brain for this particular batch.
Application Type
Pep-ELISA, WB
Selected References
[{"pmid": 26962049, "intro": "This antibody has been successfully used in IHC on Mouse:", "title": "Cross-species functional analyses reveal shared and separate roles for Sox11 in frog primary neurogenesis and mouse cortical neuronal differentiation.", "author": "Chen C, Jin J, Lee GA, Silva E, Donoghue M.", "journal": "Biol Open. 2016 Apr 15;5(4):409-17"}]