Goat Anti-SSP29 / ANP32B Antibody

$423.00

Category: Primary Antibodies
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SKU: EB05885
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
silver-stainable protein SSP29|putative HLA-DR-associated protein I-2|acidic nuclear phosphoprotein 32 family member B|acidic protein rich in leucines|acidic (leucine-rich) nuclear phosphoprotein 32 family, member B|PHAPI2|APRIL|SSP29|ANP32B
Accession ID
NP_006392.1
Blocking Peptide
EBP05885
Immunogen
Peptide with sequence C-KRKRETDDEGEDD, from the C Terminus of the protein sequence according to NP_006392.1.
Product Comments
This antibody does NOT recognize theTNF family member also known as APRIL - Gene ID number 8741. However this antibody may cross-react with ANP32A (GeneID 8125).
Peptide Sequence
C-KRKRETDDEGEDD
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Dog, Pig, Cow
Reactive Species
Human, Mouse, Rat, Pig
Human Gene ID
10541
Mouse Gene ID
67628
Rat Gene ID
170724
Product Grade
IHC Results
In paraffin embedded Human Breast shows nuclear staining in lobular cells. Recommended concentration: 3-5µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:16000.
Western Blot
Approx 28kDa band observed in Human Tonsil lysates, and approx 30kDa band observed in Rat and Pig Spleen lysates and in lysates of cell line NIH3T3 (calculated MW of 28.8kDa according to Human NP_006392.1, 31.1kDa according to Mouse NP_570959.1 and to Rat NP_571986.1 and 29.9kDa according to Pig XP_013849138.1). Recommended concentration: 0.1-0.3µg/ml.
Application Type
Pep-ELISA, WB, IHC

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