Goat Anti-STAG3 (mouse) Antibody

$255.00

Category: Primary Antibodies
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SKU: EB07815
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
stromalin 3|cohesin subunit SA-3|SCC3 homolog 3|stromal antigen 3|STAG3
Accession ID
NP_058660.2
Blocking Peptide
EBP07815
Immunogen
Peptide with sequence C-KHYNKFYEDYGD, from the internal region of the protein sequence according to NP_058660.2.
Product Comments
The immunizing peptide was designed based on the Mouse protein sequence with one residue of a diference from the human sequence.
Peptide Sequence
C-KHYNKFYEDYGD
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Mouse, Rat
Human Gene ID
10734
Mouse Gene ID
50878
Rat Gene ID
114522
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:32000.
Western Blot
Preliminary experiments gave an approx 30kDa band in Human Testis lysates after 0.5µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 141kDa according to NP_058660.2. The 30kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Application Type
Pep-ELISA

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