Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names signal transducing adaptor family member 2|signal-transducing adaptor protein-2|brk kinase substrate|breast tumor kinase substrate|FLJ20234|STAP-2|BKS|STAP2 | |
Accession ID NP_060190.2; NP_001013863.1 | |
Blocking Peptide EBP05127 | |
Immunogen Peptide with sequence C-ELQKKLEKRRALEH, from the C Terminus of the protein sequence according to NP_060190.2; NP_001013863.1. | |
Product Comments This antibody is expected to recognise isoforms 1 (NP_060190.2) and isoform 2 (NP_001013863.1). | |
Peptide Sequence C-ELQKKLEKRRALEH | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human | |
Reactive Species Human | |
Human Gene ID 55620 | |
Product Grade  | |
ELISA Detection Limit Antibody detection limit dilution 1:128000. | |
Western Blot Approx 48kDa band observed in Human Heart lysates and 45kDa band observed in Human Breast Lysates (calculated MW of 49.6kDa according to NP_060190 and of NP_001013863.1). Recommended concentration: 0.5-1.5µg/ml. | |
Application Type Pep-ELISA, WB |
Goat Anti-STAP2 / BKS Antibody
$423.00
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Selected References [{"pmid": 19393627, "intro": "This antibody (previous batch) has been successfully used in the following paper:", "title": "STAP-2 is phosphorylated at tyrosine-250 by Brk and modulates Brk-mediated STAT3 activation.", "author": "Ikeda O, Miyasaka Y, Sekine Y, Mizushima A, Muromoto R, Nanbo A, Yoshimura A, Matsuda T.", "journal": "Biochem Biophys Res Commun. 2009 Jun 19;384(1):71-5."}, {"pmid": 19933863, "intro": "This antibody (previous batch) has been successfully used in WB and IF on Human:", "title": "Signal-transducing adaptor protein-2 regulates stromal cell-derived factor-1 alpha-induced chemotaxis in T cells.", "author": "Sekine Y, Ikeda O, Tsuji S, Yamamoto C, Muromoto R, Nanbo A, Oritani K, Yoshimura A, Matsuda T.", "journal": "J Immunol. 2009 Dec 15;183(12):7966-74."}, {"pmid": 21205088, "intro": "This antibody (previous batch) has been successfully used in Western blot on Human:", "title": "Involvement of STAP-2 in Brk-mediated phosphorylation and activation of STAT5 in breast cancer cells.", "author": "Ikeda O, Mizushima A, Sekine Y, Yamamoto C, Muromoto R, Nanbo A, Oritani K, Yoshimura A, Matsuda T.", "journal": "Cancer Sci. 2011 Apr;102(4):756-61."}, {"pmid": 20929863, "intro": "This antibody (previous batch) has been successfully used in Western blot on Human:", "title": "Interactions of STAP-2 with Brk and STAT3 participate in cell growth of human breast cancer cells.", "author": "Ikeda O, Sekine Y, Mizushima A, Nakasuji M, Miyasaka Y, Yamamoto C, Muromoto R, Nanbo A, Oritani K, Yoshimura A, Matsuda T.", "journal": "J Biol Chem. 2010 Dec 3;285(49):38093-103."}] |