Goat Anti-STAT1 alpha isoform Antibody

$255.00

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SKU: EB05110
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
signal transducer and activator of transcription 1, 91kD|signal transducer and activator of transcription-1|transcription factor ISGF-3 components p91/p84|transcription factor ISGF-3|STAT91|ISGF-3|signal transducer and activator of transcription 1, 91kDa|STAT1
Usage Summary
Please note that we also get a lower size band (approx. 75kDa) in addition to the expected band. We do not have explanation for this band so far. This could represent an isoform of STAT1 (AAH02704), which has a predicted MW of 78kDa. Moreover, splice prediction software (http://www.bioinformatics.ucla.edu/ASAP/) indicated existence of constitutive splice varients for STAT1. We would appreciate any feedback from people in the field. Have any splice variants/modified forms been reported?
Accession ID
NM_007315
Blocking Peptide
EBP05110
Immunogen
Peptide with sequence C-GSVEFDSMMNTV, from the C Terminus of the protein sequence according to NM_007315.
Product Comments
This antibody is expected to recognise the alpha isoform of this protein only.
Peptide Sequence
C-GSVEFDSMMNTV
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human
Reactive Species
Human
Human Gene ID
6772
Product Grade
Western Blot
Western blot: Approx 75kDa and 90-95kDa bands seen in Jurkat cell lysates. Recommended for use at 3-5µg/ml.