Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names STAT5A| STAT5| signal transducer and activator of transcription 5A| MGF | |
Usage Summary Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
Flow Cytometry: Flow cytometric analysis of K562 cells. Recommended concentration: 10ug/ml. | |
Accession ID NP_003143.2 | |
Blocking Peptide EBP11393 | |
Immunogen Peptide with sequence C-KYYTPVLAKAVD, from the internal region of the protein sequence according to NP_003143.2. | |
Product Comments This antibody is not expected to cross-react with STAT5B. | |
Peptide Sequence C-KYYTPVLAKAVD | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human, Mouse, Rat, Dog, Cow | |
Reactive Species Human | |
Human Gene ID 6776 | |
Mouse Gene ID 20850 | |
Rat Gene ID 24918 | |
Product Grade  | |
IHC Results Paraffin embedded Human Spleen. Recommended concentration: 5µg/ml. | |
ELISA Detection Limit Antibody detection limit dilution 1:8000. | |
Western Blot Approx 100kDa band observed in lysates of cell line K562 (calculated MW of 90.6kDa according to NP_003143.2). Recommended concentration: 0.1-0.3µg/ml. Primary incubation 1 hour at room temperature | |
Application Type Pep-ELISA, WB, IHC, FC |
Goat Anti-STAT5A (aa681-692) Antibody
$464.00
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Selected References [{"pmid": 30377371, "intro": "This antibody has been successfully used in the following paper:", "title": "A high-throughput pipeline for validation of antibodies", "author": "Krzysztof Sikorski, Adi Mehta, Marit Inngjerdingen, Flourina Thakor, Simon Kling, Tomas Kalina, Tuula A. Nyman, Maria Ekman Stensland, Wei Zhou, Gustavo A. De Souza, Lars Holden, Jan Stuchly, Markus Templin and Fridtjof Lund-Johansen", "journal": "Nat Methods. 2018 Nov;15(11):909-912"}] |