Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
| |
Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names transcription factor 2|MODY5|LFB3|HNF2|HNF1beta|HNF1B|FJHN|transcription factor 2, hepatic LF-B3 variant hepatic nuclear factor|VHNF1|TCF2 | |
Usage Summary Immunocytochemistry: A customer found positive results in ICC on Human primitive gut tube from Human embryonic stem cells. | |
Accession ID NP_000449.1; NP_001159395.1 | |
Blocking Peptide EBP07588 | |
Immunogen Peptide with sequence C-QAYDRQKNPSKEER, from the internal region of the protein sequence according to NP_000449.1; NP_001159395.1. | |
Product Comments This antibody is expected to recognize both reported isoforms (NP_000449.1; NP_001159395.1). This product may cross-react with HNF1A. | |
Peptide Sequence C-QAYDRQKNPSKEER | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human, Mouse, Rat, Dog, Pig, Cow, Zebrafish | |
Reactive Species Human, Mouse | |
Human Gene ID 6928 | |
Mouse Gene ID 21410 | |
Rat Gene ID 25640 | |
Product Grade  | |
ELISA Detection Limit Antibody detection limit dilution 1:64000. | |
Western Blot Approx 60kDa band observed in Human Liver lysates (calculated MW of 61.3kDa according to NP_000449.1). Recommended concentration: 0.1-0.3µg/ml. Primary incubation was 1 hour. An additional band of unknown identity was also consistently observed at 100kDa. This band was successfully blocked by incubation with the immunising peptide.
This antibody was successfully used in WB on Mouse in the following paper: Arenas F et al. (2008) PMID: 18188457. | |
Application Type Pep-ELISA, WB, ICC |
Goat Anti-TCF2 / VHNF1 Antibody
$423.00
| SKU | Unit Size | Price |
|---|---|---|
Select a unit size:
Selected References [{"pmid": 36522156, "intro": "This antibody has been successfully used in WB in the following paper:", "title": "HNF1B Alters an Evolutionarily Conserved Nephrogenic Program of Target Genes", "author": "Kelli Grand, Martine Stoltz, Ludovica Rizzo, Ruth Röck, Michael M Kaminski, Gabriela Salinas, Maike Getwan, Thomas Naert, Roman Pichler, Soeren S Lienkamp", "journal": "J Am Soc Nephrol. 2023 Mar 1;34(3):412-432."}, {"pmid": 18188457, "intro": "This antibody has been successfully used in Western blot on Mouse:", "title": "Combination of ursodeoxycholic acid and glucocorticoids upregulates the AE2 alternate promoter in human liver cells.", "author": "Arenas F, Hervias I, Uriz M, Joplin R, Prieto J, Medina JF.", "journal": " J Clin Invest. 2008 Feb;118(2):695-709."}] |