Goat Anti-TEM8 / Anthrax Toxin Receptor 1 Antibody

$423.00

Category: Primary Antibodies
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SKU: EB06472
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
2810405N18Rik|2310008J16Rik|FLJ11298|anthrax toxin receptor 1|ANTXR1|tumor endothelial marker 8, isoform 3 precursor|tumor endothelial marker 8|FLJ21776|FLJ10601|ATR|anthrax toxin receptor|TEM8
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the membrane and cytoplasm of HeLa cells. Recommended concentration: 10µg/ml.
Accession ID
NP_115584.1
Blocking Peptide
EBP06472
Immunogen
Peptide with sequence C-RAPPPSRPPPRPSV, from the C Terminus of the protein sequence according to NP_115584.1.
Product Comments
This antibody is expected to recognise variant 1 (NP_115584) only.
Peptide Sequence
C-RAPPPSRPPPRPSV
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Rat, Dog, Cow
Reactive Species
Human
Human Gene ID
84168
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:64000.
Western Blot
Preliminary testing showed a band at approx 65-70kDa in lysate of cell lines A431 and U2OS after 1-2µg/ml antibody staining (calculated MW of 62.8kDa according to NP_115584.1). This band was successfully blocked by incubation with the immunizing peptide. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, IF

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