Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names TGFBI|transforming growth factor, beta-induced, 68kDa|RGD-containing collagen-associated protein|LCD1|kerato-epithelin|EBMD|CSD3|CSD2|CSD1|CSD|CDGG1|CDG2|CDB1|BIGH3 | |
Usage Summary Immunofluorescence: Strong expression of the protein seen in the golgi and plasma membrane of HeLa cells. Recommended concentration: 10µg/ml. | |
Accession ID NP_000349.1 | |
Blocking Peptide EBP09795 | |
Immunogen Peptide with sequence C-QLYTDRTEKLRPE, from the internal region of the protein sequence according to NP_000349.1. | |
Peptide Sequence C-QLYTDRTEKLRPE | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human, Mouse, Rat, Dog | |
Reactive Species Human, Rat | |
Human Gene ID 7045 | |
Mouse Gene ID 21810 | |
Rat Gene ID 24511 | |
Product Grade  | |
IHC Results In paraffin embedded Kidney shows staining of intercalated cells of a collecting tubule. Recommended concentration: 4-6µg/ml. This antibody has been successfully used in IHC on Rat, PMID: 26407577. | |
ELISA Detection Limit Antibody detection limit dilution 1:32000. | |
Western Blot Approx 70kDa band observed in Human and Rat Kidney lysates, and approx 75kDa in Human Prostate and Human Colorectal cancer lysates (calculated MW of 74.7kDa according to Human NP_000349.1 and 74.8kDa according to Rat NP_446254.1). Recommended concentration: 0.1-0.3µg/ml. Primary incubation 1 hour at room temperature. | |
Application Type Pep-ELISA, WB, IHC, IF |
Goat Anti-TGFBI Antibody
$464.00
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Selected References [{"pmid": 26407577, "intro": "This antibody has been successfully used in IHC on Rat:", "title": "Vaccination against type 1 angiotensin receptor prevents streptozotocin-induced diabetic nephropathy.", "author": "Dan Ding, Yimei Du, Zhihua Qiu, Sen Yan, Fen Chen, Min Wang, Shijun Yang, Yanzhao Zhou, Xiajun Hu, Yihuan Deng, Shijia Wang, Liangping Wang, Hongrong Zhang, Hailang Wu, Xian Yu, Zihua Zhou, Yuhua Liao & Xiao Chen.", "journal": "J Mol Med (Berl). 2016 Feb;94(2):207-18"}] |