Goat Anti-TIA1 Antibody

$464.00

Category: Primary Antibodies
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SKU: EB07741
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
p40-TIA-1 (containing p15-TIA-1)|cytotoxic granule-associated RNA-binding protein|TIA1 protein|TIA1 cytotoxic granule-associated RNA-binding protein|TIA1 cytotoxic granule-associated RNA binding protein|TIA1
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the nucleus and cytoplasm of HeLa cells. Recommended concentration: 5µg/ml.
Accession ID
NP_071320.1; NP_071505.1
Blocking Peptide
EBP07741
Immunogen
Peptide with sequence C-PKSTYESNTKQ, from the internal region of the protein sequence according to NP_071320.1; NP_071505.1.
Product Comments
This antibody is expected to recognise both reported isoforms (NP_071320.1 and NP_071505.1).
Peptide Sequence
C-PKSTYESNTKQ
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Dog
Reactive Species
Human
Human Gene ID
7072
Mouse Gene ID
21841
Rat Gene ID
312510
Product Grade
IHC Results
Paraffin embedded Human Thyroid. Recommended concentration: 3.75µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:128000.
Western Blot
Approx 40kDa band observed in lysates of cell line Jurkat (calculated MW of 41.8kDa according to NP_071320.1). Recommended concentration: 0.1-0.3µg/ml. Primary incubation was 1 hour. An additional band at 80kDa and a very strong 26kDa is also observed. These bands were successfully blocked by incubation with the immunising peptide.
Application Type
Pep-ELISA, WB, IF, IHC

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