Goat Anti-TIM3 / HAVCR2 Antibody

$464.00

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SKU: EB07113
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
kidney injury molecule-3|T cell immunoglobulin mucin-3|T cell immunoglobulin mucin 3|Tim-3|TIMD3|KIM-3|FLJ14428|hepatitis A virus cellular receptor 2|TIM3|HAVCR2
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the nucleus and plasma membrane of HepG2 cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.

Accession ID
NP_116171.3
Blocking Peptide
EBP07113
Immunogen
Peptide with sequence C-KWYSHSKEKIQN, from the internal region of the protein sequence according to NP_116171.3.
Peptide Sequence
C-KWYSHSKEKIQN
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Dog
Reactive Species
Human
Human Gene ID
84868
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:64000.
Western Blot
Approx 35kDa band observed in lysates of cell lines A549 and HepG2 and in preliminary testing of Jurkat and U937 cell lysate (calculated MW of 33.4kDa according to NP_116171.3). Recommended concentration:0.1-1µg/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB, IF, FC