Goat Anti-TMP21 / TMED10 Antibody

$423.00

Category: Primary Antibodies
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SKU: EB07636
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
TMED10|transmembrane emp24-like trafficking protein 10 (yeast)|P24(DELTA)|S31I125|S31III125|TMP21|Tmp-21-I|21 kDa transmembrane trafficking protein|transmembrane emp24 domain-containing protein 10
Usage Summary
Immunofluorescence:Expression of the protein seen in the Golgi and cytoplasm of Glioblastoma U251 cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Accession ID
NP_006818.3
Blocking Peptide
EBP07636
Immunogen
Peptide with sequence C-YLRRFFKAKKLIE, from the C Terminus of the protein sequence according to NP_006818.3.
Peptide Sequence
C-YLRRFFKAKKLIE
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Dog, Cow
Reactive Species
Human
Human Gene ID
10972
Mouse Gene ID
68581
Rat Gene ID
84599
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:1000.
Western Blot
Preliminary experiments gave an approx 75kDa band in Rat Pancreas lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 25.0kDa according to NP_006818.3. The 75kDa band was successfully blocked by incubation with the immunizing peptide. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, IF, FC

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