Goat Anti-TNNI2 Antibody

$423.00

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SKU: EB12036
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
troponin I, skeletal, fast|troponin I, fast-twitch skeletal muscle isoform|troponin I, fast-twitch isoform|troponin I, fast skeletal muscle|troponin I fast twitch 2|fast-twitch skeletal muscle troponin I|fsTnI|FSSV|DA2B|AMCD2B|troponin I type 2 (skeletal, fast)|TNNI2
Accession ID
NP_003273.1; NP_001139313.1
Blocking Peptide
EBP12036
Immunogen
Peptide with sequence KRNRAITARRQHLKS-C, from the N Terminus of the protein sequence according to NP_003273.1; NP_001139313.1.
Product Comments
This antibody is expected to recognize both reported isoforms (NP_003273.1; NP_001139313.1). Reported variants represent identical protein: NP_001139301.1, NP_003273.1.
Peptide Sequence
KRNRAITARRQHLKS-C
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Dog
Reactive Species
Human, Mouse, Rat
Human Gene ID
7136
Mouse Gene ID
21953
Rat Gene ID
29389
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:128000.
Western Blot
Approx. 25kDa band observed in Human, Mouse and Rat Skeletal Muscle lysates (calculated MW of 21.3kDa according to NP_003273.1). Recommended concentration: 0.03-0.1µg/ml. Primary incubation was 1 hour. This antibody has been successfully used in WB on Mouse, PMID: 34145356.
Application Type
Pep-ELISA, WB
Selected References
[{"pmid": 34145356, "intro": "This antibody has been successfully used in Western blot on Mouse:", "title": "The LIM domain protein nTRIP6 modulates the dynamics of myogenic differentiation.", "author": "Tannaz Norizadeh Abbariki, Zita Gonda, Denise Kemler, Pavel Urbanek, Tabea Wagner, Margarethe Litfn, Zhao?Qi Wang, Peter Herrlich & Olivier Kassel", "journal": "Sci Rep. 2021 Jun 18;11(1):12904."}]