Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names osm-9-like TRP channel 3|epithelial calcium channel 1|calcium transporter 2|OTRPC3|ECAC1|CAT2|transient receptor potential cation channel, subfamily V, member 5|TRPV5 | |
Usage Summary Immunocytochemsitry/Immunofluorescence: This antibody has been successfully used in ICC/IF on Human: Kennedy BG et al. (2010) PMID: 20405023. This antibody has been successfully used in ICC on Mouse: Mariana Labarca et al. (2015) PMID: 25810438. This antibody has been successfully used in ICC on Human: Tomilin VN et al. (2016) PMID: 26096460. | |
Accession ID NP_062815.2 | |
Blocking Peptide EBP08515 | |
Immunogen Peptide with sequence C-SHRGWEILRQNT, from the internal region of the protein sequence according to NP_062815.2. | |
Peptide Sequence C-SHRGWEILRQNT | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human, Mouse, Rat, Dog, Pig, Cow | |
Reactive Species Human | |
Human Gene ID 56302 | |
Mouse Gene ID 194352 | |
Rat Gene ID 116469 | |
Product Grade  | |
ELISA Detection Limit Antibody detection limit dilution 1:16000. | |
Western Blot Approx 85kDa band observed in Human Pancreas lysates (calculated MW of 82.6kDa according to NP_062815.2). Recommended concentration: 1-3µg/ml. Primary incubation was 1 hour. This antibody has been successfully used in WB on Human: Kennedy BG et al. (2010) PMID: 20405023. | |
Application Type Pep-ELISA, WB, ICC, IF |
Goat Anti-TRPV5 Antibody
$464.00
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Selected References [{"pmid": 25810438, "intro": "This antibody has been successfully used in ICC on Mouse:", "title": "Harvest and primary culture of the murine aldosterone-sensitive distal nephron", "author": "Mariana Labarca, Jonathan M. Nizar, Elisabeth M. Walczak, Wuxing Dong, Alan C. Pao and Vivek Bhalla", "journal": "Am J Physiol Renal Physiol. 2015 Jun 1; 308(11)"}, {"pmid": 20405023, "intro": "This antibody has been successfully used in WB and ICC in Human:", "title": "Expression of transient receptor potential vanilloid channels TRPV5 and TRPV6 in retinal pigment epithelium.", "author": "Kennedy BG, Torabi AJ, Kurzawa R, Echtenkamp SF, Mangini NJ.", "journal": "Mol Vis. 2010 Apr 14;16:665-75."}, {"pmid": 26096460, "intro": "This antibody has been successfully used in IF in Human:", "title": "TRPV5/V6 Channels Mediate Ca(2+) Influx in Jurkat T Cells Under the Control of Extracellular pH.", "author": "Tomilin VN, Cherezova AL, Negulyaev YA, Semenova SB.", "journal": "J Cell Biochem. 2016 Jan;117(1):197-206"}] |