Goat Anti-Tryptophan hydroxylase 2 / TPH2 Antibody

$464.00

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SKU: EB07050
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
tryptophan 5-monooxygenase 2|MGC138872|MGC138871|ADHD7|neuronal tryptophan hydroxylase|NTPH|FLJ37295|HGNC:20692|tryptophan hydroxylase 2|TPH2
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U251 and A549 cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of U251 cells. Recommended concentration: 10ug/ml.

Accession ID
NP_775489.2
Blocking Peptide
EBP07050
Immunogen
Peptide with sequence C-NKPNSGKNDDKGNK, from the internal region of the protein sequence according to NP_775489.2.
Peptide Sequence
C-NKPNSGKNDDKGNK
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Dog, Pig, Cow
Reactive Species
Human
Human Gene ID
121278
Product Grade
IHC Results
In paraffin embedded Human Brain Stem shows staining of the cytoplasm of Purkinje cells and of neuron projections. Recommended concentration, 2-4µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:32000.
Application Type
Pep-ELISA, FC, IF, IHC
Selected References
[{"pmid": 27514574, "intro": "This antibody (previous batch) has been successfully used in IF in Mouse:", "title": "Mitochondrial Changes and Oxidative Stress in a Mouse Model of Zellweger Syndrome Neuropathogenesis.", "author": "Rani Sadia Rahim, Mo Chen, C. Cathrin Nourse, Adrian C. B. Meedeniya and Denis I. Crane.", "journal": "Neuroscience 334 (2016) 201–213."}, {"pmid": 29187321, "intro": "This antibody (previous batch) has been successfully used in IF on Mouse:", "title": "Impaired neurogenesis and associated gliosis in mouse brain with PEX13 deficiency.", "author": "Rahim RS, St John JA, Crane DI, Meedeniya ACB", "journal": "Mol Cell Neurosci. 2018 Apr;88:16-32"}]