Goat Anti-UBE2L3 Antibody

$423.00

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SKU: EB05770
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
ubiquitin-protein ligase|ubiquitin-conjugating enzyme UBCH7|ubiquitin carrier protein|UbcM4|L-UBC|E2-F1|UBCH7|ubiquitin-conjugating enzyme E2L 3|UBE2L3
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the cytoplasm. Dividing cells appear to show enhanced expression. Recommended concentration: 5µg/ml.
Accession ID
NP_003338.1
Blocking Peptide
EBP05770
Immunogen
Peptide with sequence C-EFTKKYGEKRPVD, from the C Terminus of the protein sequence according to NP_003338.1.
Peptide Sequence
C-EFTKKYGEKRPVD
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Pig, Cow
Reactive Species
Human, Mouse, Rat, Pig
Human Gene ID
7332
Mouse Gene ID
22195
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:128000.
Western Blot
Approx 18kDa band observed in lysates of cell lines HeLa HEK293, HepG2 and Jurkat and also in lysates of Human, Mouse and Rat Heart, Skeletal Muscle and Testis and of Pig Heart (calculated MW of 17.9kDa according to Human NP_003338.1 and Mouse NP_033482.1). Recommended concentration: 1-3µg/ml.
Application Type
Pep-ELISA, WB, IF