Goat Anti-VPS18 Antibody

$255.00

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SKU: EB06829
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
PEP3|vacuolar protein sorting 18 homolog (S. cerevisiae)|vacuolar protein sorting 18|HGNC:15972, KIAA1475|vacuolar protein sorting protein 18|VPS18
Accession ID
NP_065908.1
Blocking Peptide
EBP06829
Immunogen
Peptide with sequence CADLPEEDEELRKK, from the internal region of the protein sequence according to NP_065908.1.
Peptide Sequence
CADLPEEDEELRKK
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Dog, Pig, Cow
Human Gene ID
57617
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:16000.
Western Blot
Preliminary experiments gave an approx 25-26kDa band in lysates of Human Duodenum, Colon and Placenta after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 110kDa according to NP_065908.1. The 25-26kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Application Type
Pep-ELISA