Goat Anti-ZIC2 Antibody

$423.00

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SKU: EB10810
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
zinc finger protein ZIC 2| Zinc finger protein of the cerebellum 2| Zic family member 2 (odd-paired Drosophila homolog)| HPE5| Zic family member 2 (odd-paired homolog, Drosophila)|ZIC2
Accession ID
NP_009060.2
Blocking Peptide
EBP10810
Immunogen
Peptide with sequence C-HSGLSSNFNEWY, from the C Terminus of the protein sequence according to NP_009060.2.
Product Comments
This antibody is expected to NOT cross-react with other ZIC proteins.
Peptide Sequence
C-HSGLSSNFNEWY
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Dog, Cow
Reactive Species
Human
Human Gene ID
7546
Mouse Gene ID
22772
Rat Gene ID
361096
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:1000.
Western Blot
In transfected RWPE1 transiently expressing Human ZIC2-Myc a band of approx. 65kDa is observed. This band is not observed in the non-transfected RWPE1, nor when GFP is transiently expressed (calculated Mwt. of 55.0kDa according to NP_009060.2 and 58.6kDa for the ZIC2-Myc protein). Recommended concentration, 0.5-2µg/ml. Primary incubation overnight at 4C Data kindly provided by Nathan Bowen and Maryam Boseman , Clark Atlanta University, GA, USA..
Application Type
Pep-ELISA, WB