Goat Anti-ZIC3 (aa178-189) Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

zinc finger protein of the cerebellum 3| zinc finger protein ZIC 3| heterotaxy 1| ZNF203| HTX1| HTX| Zic family member 3 (odd-paired homolog, Drosophila)|ZIC3

NP_003404.1

EBP10811

Peptide with sequence C-HVDNNQVHLGLR, from the internal region of the protein sequence according to NP_003404.1.

This antibody is expected to NOT cross-react with other ZIC proteins.

C-HVDNNQVHLGLR

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Human, Mouse, Rat, Dog, Pig, Cow

Human

7547

22773

367944

In paraffin embedded Human Prostate shows nuclear staining in select secretory cells. Recommended concentration, 5-10µg/ml.

Antibody detection limit dilution 1:64000.

Preliminary experiments gave an approx 70kDa band in Human, Mouse and Rat Brain lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 50.6kDa according to NP_003404.1. The 70kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Pep-ELISA, IHC