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Goat Anti-Tankyrase / TANK1 Antibody (EB02508)

Code Name Applications Availability Size Tested species Price Grade  
EB02508 Goat Anti-Tankyrase / TANK1 Antibody WB-Trf Yes 100µg specific antibody in 200µl Human $423.00 Elite

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EB02508 1
HEK293 overexpressing TANK1 (lane 2) and TANK2 (lane 4) and probed with EB02508 (mock transfection in first lane). Lane three is empty. Lower panel shows the same lysates probed for alpha-Actin to show protein levels. Primary incubation (0.5ug/ml) was overnight at 4°C. Detected by chemiluminescence.
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Target Protein

Principal Names: TNKS, tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase, HGNC:11941, PARP-5a, PARP5A, PARPL, TIN1, TINF1, TNKS1
Official Symbol: TNKS
Accession Number(s): NP_003738.2
Human GeneID(s): 8658
Important Comments: Fusion protein containing 120aa amino acids from N Terminal region (according to NP_003738.2)

Immunogen

Fusion protein containing 120aa amino acids from N Terminal region of the protein sequence according to NP_003738.2. No cross reactivity expected to TANK2.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Western blot: In transfected HEK293 transiently expressing TANK1 a band of approx. 142kDa is observed. This band is not observed in the non-transfected HEK293. The calculated molecular size is 142 kDa according to NP_003738.2. Recommended concentration, 0.5-1.5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human