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Goat Anti-STAP2 / BKS (aa391-403) Antibody (EB05074)

Related Products: EBP05074 - Immunizing Peptide, EBP05127 - Immunizing Peptide, Goat Anti-STAP2 / BKS Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB05074 Goat Anti-STAP2 / BKS (aa391-403) Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB05074 1
EB05074 (0.3µg/ml) staining of Human Breast lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB05074 2
EB05074 (3.75µg/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: STAP2, signal transducing adaptor family member 2, BKS, FLJ20234, brk kinase substrate, BRK substrate, breast tumor kinase substrate, brk kinase substrate, signal-transducing adaptor protein 2, signal-transducing adaptor protein-2
Official Symbol: STAP2
Accession Number(s): NP_060190.2; NP_001013863.1
Human GeneID(s): 55620
Important Comments: This antibody is expected to recognize isoform 1 (NP_060190.2) and isoform 2 (NP_001013863.1).

Immunogen

Peptide with sequence C-LQKKLEKRRALEH, from the C Terminus of the protein sequence according to NP_060190.2; NP_001013863.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 49kDa band observed in Human Breast lysates (calculated MW of 49.6kDa according to NP_060190.2). Recommended concentration: 0.3-1µg/ml.
IHC: Paraffin embedded Human Small Intestine. Recommended concentration: 3.75µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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