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Goat Anti-SHP2 / PTPN11 Antibody (EB05114)

Related Products: EBP05114 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB05114 Goat Anti-SHP2 / PTPN11 Antibody Pep-ELISA, WB, IF, FC Yes 100µg specific antibody in 200µl Human $450.00 Elite_Plus

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ARP American Research Products, Inc
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EB05114 1
EB05114 staining (2µg/ml) of Human Muscle lysate (RIPA buffer, 35µg total protein per lane). Detected by chemiluminescence.
EB05114 2
EB05114 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB05114 3
EB05114 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: PTPN11, SHP2, protein tyrosine phosphatase, non-receptor type 11 (Noonan syndrome 1), CFC, NS1, BPTP3, PTP2C, SHP-2, PTP-1D, SH-PTP2, SH-PTP3, Noonan syndrome 1, protein-tyrosine phosphatase 2C, protein tyrosine phosphatase, non-receptor type 11, MGC14433, protein tyrosine phosphatase-2
Official Symbol: PTPN11
Accession Number(s): NP_002825.3; NP_001317366.1
Human GeneID(s): 5781
Non-Human GeneID(s): 19247 (mouse), 25622 (rat)

Immunogen

Peptide with sequence C-YENVGLMQQQKSFR, from the C Terminus of the protein sequence according to NP_002825.3; NP_001317366.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 70kDa band observed in Human Muscle lysates (calculated MW of 68.0kDa according to NP_002825.3). Recommended concentration: 1-3µg/ml. Primary incubation 1 hour at room temperature.

Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of HeLa cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Pig, Cow

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