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Goat Anti-FYN Antibody (EB05153)

Related Products: EBP05153 - Immunizing Peptide, Goat Anti-FYN Antibody - Trial
Code Name Applications Availability Size Tested species Price Grade  
EB05153 Goat Anti-FYN Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB05153 1
EB05153 (0.3µg/ml) staining of A431 (A) and Jurkat lysate (B) (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: FYN, SLK, SYN, MGC45350, FYN oncogene related to SRC, FGR, YES, src-like kinase, c-syn protooncogene, tyrosine kinase p59fyn(T), src/yes-related novel gene, OKT3-induced calcium influx regulator, proto-oncogene tyrosine-protein kinase fyn, RP1-66H14.1, OTTHUMP00000017917, protein-tyrosine kinase fyn, src-like kinase
Official Symbol: FYN
Accession Number(s): NP_002028.1; NP_694592.1; NP_694593.1
Human GeneID(s): 2534
Non-Human GeneID(s): 14360 (mouse), 25150 (rat)
Important Comments: This antibody is expected to recognise all three reported isoforms (as represented by NP_002028.1; NP_694592.1; NP_694593.1).

Immunogen

Peptide with sequence VQCKDKEATKLTE, from the N Terminus of the protein sequence according to NP_002028.1; NP_694592.1; NP_694593.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 55kDa band observed in lysates of cell lines A431 and Jurkat (calculated MW of 54.5kDa according to NP_694593.1). Recommended for use at 0.3-1µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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