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Goat Anti-PRAM1 Antibody (EB05159)

Related Products: EBP05159 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB05159 Goat Anti-PRAM1 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB05159 1
EB05159 staining (2µg/ml) of Jurkat lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 12 hour. Detected by western blot using chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: PRAM-1, PRAM-1 protein, PML-RARA target gene encoding an Adaptor Molecule-1, PML-RARA regulated adaptor molecule 1, MGC39864, PML-RAR
Official Symbol: PRAM1
Accession Number(s): NP_115528.4
Human GeneID(s): 84106

Immunogen

Peptide with sequence DFCDPLENQPLPLGR, from the C Terminus of the protein sequence according to NP_115528.4.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx 90-100kDa band seen in Jurkat cell lysates. Recommended for use at 1-3µg/ml. Overnight incubation is recommended with this antibody. Please note that although the predicted MW of PRAM-1 is 79kDa, Moog-Lutz et al., (J. Biol. Chem, Jun 2001; 276: 22375 - 22381) detected aPRAM-1 migrating with an apparent molecular mass of 97 kDa and this is what we observed as well.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Pig

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