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Goat Anti-PTP4A1 / PRL-1 Antibody (EB05243)

Code Name Applications Availability Size Tested species Price Grade  
EB05243 Goat Anti-PTP4A1 / PRL-1 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 

EB05243 1
EB05243 (1µg/ml) staining of A549 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: PTP4A1, PRL-1, protein tyrosine phosphatase type IVA, member 1, HH72, PRL1, PTP4A2, PTPCAAX1, PTP(CAAX1), Protein tyrosine phosphatase IVA1, RP5-1148A21.2, DKFZp779M0721, protein tyrosine phosphatase type IVA protein 1
Official Symbol: PTP4A1
Accession Number(s): NP_003454.1
Human GeneID(s): 7803

Immunogen

Peptide with sequence KDSNGHRNNCCIQ, from the C Terminus of the protein sequence according to NP_003454.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:2000.
Western blot: Approx 21kDa band observed in lysates of cell lines A549 and HeLa (calculated MW of 19.8kDa according to NP_003454.1). Recommended concentration: 1-3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog

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FAQs

Q: Is there cross-reactivity with PRL3?

A: No, a BLAST search of the immunizing peptide reveals no match with any other human protein.