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Goat Anti-ATF5 Antibody (EB05246)

Related Products: EBP05246 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB05246 Goat Anti-ATF5 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse, Rat $411.00 Elite

Ordering Information for

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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  • Purchase order number
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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB05246 1
EB05246 (1.5µg/ml) staining of Human Heart lyssate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB05246 2
EB05246 (1µg/ml) staining of Mouse (A) and Rat (B) Skeletal Muscle lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: ATF5, activating transcription factor 5, ATFX, FLJ34666, HMFN0395
Official Symbol: ATF5
Accession Number(s): NP_036200.2
Human GeneID(s): 22809
Non-Human GeneID(s): 107503 (mouse), 282840 (rat)

Immunogen

Peptide with sequence EVYKARSQRTRSC, from the C Terminus of the protein sequence according to NP_036200.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 29kDa band observed in Human Heart lysates (calculated MW of 30.7kDa according to NP_036200.2). Recommended concentration: 1-3µg/ml.

Species Reactivity

Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat

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