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Goat Anti-COX1 / PTGS1 Antibody (EB05285)

Related Products: EBP05285 - Immunizing Peptide, Goat Anti-COX1 / PTGS1 Antibody - Trial
Code Name Applications Availability Size Tested species Price Grade  
EB05285 Goat Anti-COX1 / PTGS1 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB05285 1
EB05285 (1µg/ml) staining of U937 cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB05285 2
EB05285 (3.8µg/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: PTGS1, COX1, COX3, PHS1, PCOX1, PGHS1, PTGHS, PGG/HS, PGHS-1, prostaglandin-endoperoxide synthase 1 (prostaglandin G/H synthase and cyclooxygenase), prostaglandin G/H synthase and cyclooxygenase, RP11-542K23.6
Official Symbol: PTGS1
Accession Number(s): NP_000953.2; NP_542158.1
Human GeneID(s): 5742
Important Comments: This antibody is expected to recognise both human isoforms of this protein according to NP_000953.2 and NP_542158.1.

Immunogen

Peptide with sequence C-QDDGPAVERPSTEL, from the C Terminus of the protein sequence according to NP_000953.2; NP_542158.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 70kDa band observed in lysates of histiocytic lymphoma cell line U937 (calculated MW of 68.7kDa according to NP_000953.2) Recommended for use at 0.3-1µg/ml. Primary incubation 1 hour at room temperature.
IHC: In paraffin embedded Human Heart shows patchy staining of myocardial fibres in longitudinal section. Recommended concentration, 3-5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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