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Goat Anti-DKK4 Antibody (EB05362)

Related Products: EBP05362 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB05362 Goat Anti-DKK4 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB05362 1
EB05362 staining (1µg/ml) of Jurkat lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: DKK4, dickkopf homolog 4 (Xenopus laevis), DKK-4, hdkk-4, Dickkopf gene 4, dickkopf (Xenopus laevis) homolog 4, MGC129562, MGC129563, dickkopf homolog 4
Official Symbol: DKK4
Accession Number(s): NP_055235.1
Human GeneID(s): 27121

Immunogen

Peptide with sequence RQHARLRVCQKIEKL, from the C Terminus of the protein sequence according to NP_055235.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 30-35kDa band seen in Jurkat lysate. Recommended for use at 1-3µg/ml. Please note that literature indicates DKK4 is detected as three major immunoreactive species of approx. 40 kDa [form (i)], 30-32 kDa [form (ii)] and 15-17 kDa [form (iii)]. We believe this antibody will recognise 30-32kDa species of DKK4.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig

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