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Goat Anti-WNT4 Antibody (EB05374)

Related Products: EBP05374 - Immunizing Peptide, Goat Anti-WNT4 Antibody - Trial
Code Name Applications Availability Size Tested species Price Grade  
EB05374 Goat Anti-WNT4 Antibody Pep-ELISA, WB, IF Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
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EB05374 1
EB05374 (1µg/ml) staining of Jurkat (A) and negative control U937 (B) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB05374 2
EB05374 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).
EB05374 3
EB05374 Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing vesicle staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).
Click here to download PDF datasheet.

Target Protein

Principal Names: WNT4, wingless-type MMTV integration site family, member 4, WNT-4, WNT-4 protein precursor, RP1-224A6.7, SERKAL, WNT-4 protein
Official Symbol: WNT4
Accession Number(s): NP_110388.2
Human GeneID(s): 54361
Non-Human GeneID(s): 22417 (mouse)

Immunogen

Peptide with sequence C-SNWLYLAKLSSVGS, from the internal region of the protein sequence according to NP_110388.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:4000.
Western blot: Approx 40+35kDa band observed in lysates of cell line Jurkat (calculated MW of 39.1kDa according to NP_110388.2). Recommended concentration: 1-3µg/ml. Primary incubation 1 hour at room temperature. Negative Control: U937 cell lysate.

Immunofluorescence: Strong expression of the protein seen in the Vesicle of HeLa and MCF7 cells. Recommended concentration: 10µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat

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