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Goat Anti-IRF6 Antibody (EB05404)

Related Products: EBP05404 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB05404 Goat Anti-IRF6 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

Ordering Information for

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB05404 1
EB05404 staining (2µg/ml) of Human Ovary (A) and Kidney (B) lysate (RIPA buffer, 35µg total protein per lane). Detected by chemiluminescence.
EB05404 2
EB05404 (5µg/ml) staining of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
EB05404 3
EB05404 Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: IRF6, interferon regulatory factor 6, LPS, PIT, PPS, VWS, OFC6
Official Symbol: IRF6
Accession Number(s): NP_006138.1
Human GeneID(s): 3664
Non-Human GeneID(s): 54139 (mouse)

Immunogen

Peptide with sequence C-TPSMQLPPALPPQ, from the C Terminus of the protein sequence according to NP_006138.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:4000.
Western blot: Approx 58-60kDa band observed in Human Ovary and approx. 60kDa in Human Kidney lysates (calculated MW of 53.1kDa according to NP_006138.1).The observed molecular weight corresponds to different antibodies from other commercial sources. Recommended concentration: 2-3µg/ml. Primary incubation 1 hour at room temperature.
IHC: Paraffin embedded Human Kidney. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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