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Goat Anti-MRGX Antibody (EB05410)

Related Products: EBP05410 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB05410 Goat Anti-MRGX Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB05410 1
EB05410 staining (0.2µg/ml) of A431 lysate (RIPA buffer, 35µg total protein per lane). Detected by chemiluminescence.
EB05410 2
HEK293 overexpressing MORF4L2 (RC210566) and probed with EB05410 (mock transfection in first lane), tested by Origene.
Click here to download PDF datasheet.

Target Protein

Principal Names: MORF4L2, MRGX, MORF-related gene X, KIAA0026, KIAA0026 gene product, mortality factor 4 like 2, RP5-1055C14.2, MORFL2, MSL3-2 protein, OTTHUMP00000023756
Official Symbol: MORF4L2
Accession Number(s): NP_036418.1
Human GeneID(s): 9643
Non-Human GeneID(s): 56397 (mouse)

Immunogen

Peptide with sequence SSRKQGSQPRGQQS-C, from the N Terminus of the protein sequence according to NP_036418.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 35kDa band observed in lysates of cell line A431 (calculated MW of 32.3kDa according to NP_036418.1). In transfected HEK293 transiently expressing MORF4L2 a band of approx. 37kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 1-3µg/ml. Primary incubation was for 1 hour.

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

Specific Reference

This antibody has been successfully used in the following paper:
Krzysztof Sikorski, Adi Mehta, Marit Inngjerdingen, Flourina Thakor, Simon Kling, Tomas Kalina, Tuula A. Nyman, Maria Ekman Stensland, Wei Zhou, Gustavo A. De Souza, Lars Holden, Jan Stuchly, Markus Templin and Fridtjof Lund-Johansen
A high-throughput pipeline for validation of antibodies
Nat Methods. 2018 Nov;15(11):909-912
PMID: 30377371

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