Choose your country here (United States of America)
Search
Type in product code or name:
Goat Anti-SAP130 / SF3B3 Antibody (EB05543)
| Code | Name | Applications | Availability | Size | Tested species | Price | Grade | |
|---|---|---|---|---|---|---|---|---|
| EB05543 | Goat Anti-SAP130 / SF3B3 Antibody | Pep-ELISA, WB |  |
100µg specific antibody in 200µl | Human | $423.00 |  |
|
Ordering Information for United States of America (change)Order direct from Everest
$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories. |
||||||||
EB05543 staining (0.1µg/ml) of nuclear lysate from HeLa cells (RIPA buffer, 30µg total protein per lane). Detected by chemiluminescence.
Target Protein
Principal Names: SF3B3, SAP130, splicing factor 3b, subunit 3, 130kDa, RSE1, SF3B130, KIAA0017, STAF130, pre-mRNA splicing factor SF3b, 130 kDa subunit, spliceosome-associated protein 130, splicing factor 3b, subunit 3, SF3b130Official Symbol: SF3B3
Accession Number(s): NP_036558.3
Human GeneID(s): 23450
Non-Human GeneID(s): 101943 (mouse)
Immunogen
Peptide with sequence C-VSKKLEDIRTRYAF, from the C Terminus of the protein sequence according to NP_036558.3.
Please note the peptide is available for sale.
Purification and Storage
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.
Applications Tested
Peptide ELISA: antibody detection limit dilution 1:2000.Western blot: Approx 150kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 136kDa according to NP_036558.3). Recommended concentration: 0.1-0.3µg/ml. Preliminary testing was unsuccessful on NIH3T3 for this particular batch. Primary incubated for 1 hour.
Species Reactivity
Tested: HumanExpected from sequence similarity: Human, Mouse, Rat, Dog, Cow