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Goat Anti-GATA3 Antibody (EB05570)

Code Name Applications Availability Size Tested species Price Grade  
EB05570 Goat Anti-GATA3 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 

EB05570 1
EB05570 (0.5µg/ml) staining of Human Tonsil (A) and Mouse Kidney (B) lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence
Click here to download PDF datasheet.

Target Protein

Principal Names: GATA3, GATA binding protein 3, HDR, MGC2346, MGC5199, MGC5445, GATA-binding protein 3, enhancer-binding protein GATA3
Official Symbol: GATA3
Accession Number(s): NP_001002295.1; NP_002042.1
Human GeneID(s): 2625
Non-Human GeneID(s): 14462 (mouse)
Important Comments: This antibody is expected to recognize both reported isoforms (NP_001002295.1 and NP_002042.1).

Immunogen

Peptide with sequence EVTADQPRWVSHH-C, from the N Terminus of the protein sequence according to NP_001002295.1; NP_002042.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 46kDa band observed in Human Tonsil and in Mouse Kidney lysates. (calculated MW of 47.9kDa according to NP_002042.1 and Mouse NP_032117.1 ). Recommended concentration: 0.3-1µg/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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