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Goat Anti-GATA1 Antibody (EB05571)

Related Products: EBP05571 - Immunizing Peptide, EBP06874 - Immunizing Peptide, Goat Anti-GATA1 Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB05571 Goat Anti-GATA1 Antibody Pep-ELISA, WB, IF, FC Yes 100µg specific antibody in 200µl Human $450.00 Elite_Plus

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB05571 1
EB05571 (2µg/ml) staining of K562 nuclear cell lysate (A) + peptide (B) and negative control Human Hippocampus (C) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB05571 2
EB05571 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB05571 3
EB05571 Flow cytometric analysis of paraformaldehyde fixed K562 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: GATA1, GATA binding protein 1 (globin transcription factor 1), GF1, NFE1, ERYF1
Official Symbol: GATA1
Accession Number(s): NP_002040.1
Human GeneID(s): 2623

Immunogen

Peptide with sequence C-PTTSTTVVAPLSS, from the C Terminus of the protein sequence according to NP_002040.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 50kDa band observed in nuclear lysates of cell line K562 (calculated MW of 42.8kDa according to NP_002040.1). The observed molecular weight corresponds to different antibodies from other sources and was successfully blocked by incubation with the immunizing peptide. Recommended concentration: 1-3µg/ml. Primary incubation 1 hour at room temperature. Negative Control: Human Hippocampus lysate.

Immunofluorescence: Strong expression of the protein seen in the nuclei of HeLa cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of K562 cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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