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Goat Anti-RAB11A / YL8 Antibody (EB05574)

Related Products: EBP05574 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB05574 Goat Anti-RAB11A / YL8 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Mouse $411.00 Elite

Ordering Information for

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB05574 1
EB05574 staining (2µg/ml) of mouse heart lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
EB05574 2
HEK293 overexpressing RAB11A(RC200352) and probed with EB05574 (mock transfection in first lane), tested by Origene.
Click here to download PDF datasheet.

Target Protein

Principal Names: RAB11A, YL8, RAB11A, member RAS oncogene family, RAB 11A, member oncogene family, MGC1490, Ras-related protein Rab-11A
Official Symbol: RAB11A
Accession Number(s): NP_004654.1
Human GeneID(s): 8766
Non-Human GeneID(s): 53869 (mouse), 81830 (rat)

Immunogen

Peptide with sequence TENKPKVQCCQNI, from the C Terminus of the protein sequence according to NP_004654.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 26kDa band observed in Mouse Heart lysates (calculated MW of 24.4kDa according to NP_004654.1). In transfected HEK293 transiently expressing RAB11A a band of approx. 28kDa is observed. This band is not observed in the non-transfected HEK293. Recommended for use at 1-3µg/ml.

Species Reactivity

Tested: Mouse
Expected from sequence similarity: Human, Mouse, Rat, Cow

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