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Goat Anti-MAD4 / MXD4 Antibody (EB05652)

Code Name Applications Availability Size Tested species Price Grade  
EB05652 Goat Anti-MAD4 / MXD4 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $423.00 Elite

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EB05652 1
EB05652 staining (0.5µg/ml) of human kidney lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: MXD4, MAD4, MAX dimerization protein 4, Mad4 homolog, MST149, MSTP149, bHLHc12
Official Symbol: MXD4
Accession Number(s): NP_006445.1
Human GeneID(s): 10608

Immunogen

Peptide with sequence GPHCRRLGRPALS, from the C Terminus of the protein sequence according to NP_006445.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 22kDa band observed in human kidney lysates (predicted MW of 24kDa according to NP_006445). Recommended for use at 0.5-2µg/ml. Please note the appearance of this band was blocked by incubation with the immunising peptide.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human