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Code Name Applications Availability Size Tested species Price Grade  
EB05707 Goat Anti-NDRG1 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB05707 1
EB05707 (0.1µg/ml) staining of Human Kidney (A), Cerebellum (B) and Testis (C) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB05707 2
EB05707 (0.5µg/ml) staining of paraffin embedded Human hepatocelluar carcinoma. Data obtained from C. Furer, Visceral Surgery, University of Bern, Switzerland.
EB05707 3
EB05707 (2.5µg/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB05707 4
EB05707 (2.5µg/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: NDRG1, N-myc downstream regulated gene 1, CAP43,, CMT4D, DRG1, GC4, HMSNL, NDR1, NMSL, PROXY1, RIT42, RTP, TARG1, TDD5, N-myc downstream regulated gene 1 protein, differentiation-related gene 1 protein, nickel-specific induction protein Cap43, protein regulated by oxygen-1, reducing agents and tunicamycin-responsive protein, tunicamycin-responsive protein, DRG-1
Official Symbol: NDRG1
Accession Number(s): NP_006087.2
Human GeneID(s): 10397
Non-Human GeneID(s): 17990 (mouse)
Important Comments: The variants (NP_006087.2; NP_001128714.1) encode the same protein

Immunogen

Peptide with sequence GNSAGPKSMEVSC, from the C Terminus of the protein sequence according to NP_006087.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 48kDa band observed in Human Kidney, and approx 50kDa band observed in Human Brain (Cerebellum) and Testis lysates (calculated MW of 42.8kDa according to NP_006087.2). This molecular weight is routinely observed by other sources. Recommended concentration: 0.1-0.3µg/ml. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Mouse and Rat for this particular batch.
IHC: In paraffin embedded Human hepatocelluar carcinoma shows strong labeling of membranes and weaker staining of cytoplasm. These observations are consistent with those obtained by different antibodies. Recommended concentration: 0.5-2µg/ml. Paraffin embedded Human Prostate and Colon. Recommended concentration: 2.5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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