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Goat Anti-PRDM1 / BLIMP1 Antibody (EB05710)

Related Products: EBP05710 - Immunizing Peptide, EBP09715 - Immunizing Peptide, Goat Anti-PRDM1 / MEL1 Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB05710 Goat Anti-PRDM1 / BLIMP1 Antibody Pep-ELISA, IF, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB05710 1
EB05710 Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB05710 2
EB05710 (6µg/ml) staining of paraffin embedded Human Tonsil. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
EB05710 3
EB05710 Negative Control showing staining of paraffin embedded Human Tonsil, with no primary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: PRDM1, PR domain containing 1, with ZNF domain, RP1-134E15.1, BLIMP1, MGC118922, MGC118923, MGC118924, MGC118925, PRDI-BF1, B-lymphocyte-induced maturation protein 1, PR domain containing 1, with ZNF domain, isoform 2, PR-domain zinc finger protein 1, PRDI-binding factor-1, beta-interferon gene positive-regulatory domain I binding factor, positive regulatory domain I-binding factor 1
Official Symbol: PRDM1
Accession Number(s): NP_001189.2; NP_878911.1
Human GeneID(s): 639
Non-Human GeneID(s): 12142 (mouse)
Important Comments: This antibody is expected to recognize both reported isoforms (NP_001189.2 and NP_878911.1).

Immunogen

Peptide with sequence C-KVKQETVEPMDP, from the C Terminus of the protein sequence according to NP_001189.2; NP_878911.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:2000.
IHC: Paraffin embedded Human Tonsil. Recommended concentration: 5-6µg/ml.

Immunofluorescence: Strong expression of the protein seen in the nucleus and plasma membrane of U251 cells. Recommended concentration: 10µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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