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Goat Anti-ABCB9 / TAPL Antibody (EB05736)

Related Products: EBP05736 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB05736 Goat Anti-ABCB9 / TAPL Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB05736 1
EB05736 (3.8µg/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: ABCB9, ATP-binding cassette, sub-family B (MDR/TAP), member 9, TAPL, KIAA1520, EST122234
Official Symbol: ABCB9
Accession Number(s): NP_062570.1; NP_062571.1; NP_982269.1
Human GeneID(s): 23457
Important Comments: This antibody is expected to recognise both human isoforms of this protein (as represented by NP_062570.1, NP_062571.1and NP_982269.1). Variants (NP_062571.1; NP_982269.1) encode the same isoform.

Immunogen

Peptide with sequence C-GHNEPVANGSHKA, from the C Terminus of the protein sequence according to NP_062570.1; NP_062571.1; NP_982269.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Preliminary experiments gave no signal but low background in human brain and liver lysates at up to 1µg/ml. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?
IHC: In paraffin embedded Human Testis shows vesiculate cytoplasm staining of primary spermatocytes. Recommended concentration, 3-5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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