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Goat Anti-ZIC5 Antibody (EB05745)

Related Products: EBP05745 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB05745 Goat Anti-ZIC5 Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB05745 1
EB05745 (3.75µg/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB05745 2
EB05745 (3.75µg/ml) staining of paraffin embedded Human Skin. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB05745 3
EB05745 (3.75µg/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: ZIC5, zinc finger protein of the cerebellum 5, zinc family member 5 protein, Zic family member 5 (odd-paired homolog, Drosophila)
Official Symbol: ZIC5
Accession Number(s): NP_149123.2
Human GeneID(s): 85416

Immunogen

Peptide with sequence C-EIYGNPEVVRTIH, from the C Terminus of the protein sequence according to NP_149123.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Preliminary experiments gave an approx 45kDa band in human brain lysates at 1ug/ml. This band was successfully blocked by incubation with the immunising peptide. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of approx. 73kDda according to NP_149123.
IHC: Paraffin embedded Human Small Intestine, Skin and Prostate. Recommended concentration: 3.75µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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