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Goat Anti-UBC3B Antibody (EB05752)

Related Products: EBP05752 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB05752 Goat Anti-UBC3B Antibody Pep-ELISA, IEM, IHC Yes 100µg specific antibody in 200µl Human, Rat $411.00 Elite

Ordering Information for

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB05752 1
EB05752 (2µg/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: UBE2R2, UBC3B, CDC34B, FLJ20419, MGC10481, ubiquitin-conjugating enzyme E2R 2, ubiquitin-conjugating enzyme UBC3B
Official Symbol: UBE2R2
Accession Number(s): NP_060281
Human GeneID(s): 54926
Non-Human GeneID(s): 67615 (mouse)

Immunogen

Peptide with sequence AQQQMTSSQKALM-C, from the N Terminus of the protein sequence according to NP_060281.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:4000.
Western blot: No signal obtained yet but low background observed in Human Testis and U937 lysates at up to 1µg/ml. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?
IHC: In paraffin embedded Human Testis shows textured nuclear staining of the primary spermatocytes . Recommended concentration, 2-4µg/ml.

Immunoelectron microscopy: This product stained chromatoid bodies in Rat spermatids.

Species Reactivity

Tested: Human, Rat
Expected from sequence similarity: Human, Mouse, Rat

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