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Goat Anti-BAZ2B Antibody (EB05790)

Related Products: EBP05790 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB05790 Goat Anti-BAZ2B Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB05790 1
EB05790 (5µg/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: BAZ2B, bromodomain adjacent to zinc finger domain, 2B, DKFZP434H071, DKFZp762I0516, FLJ45644, WALp4
Official Symbol: BAZ2B
Accession Number(s): NP_038478.2
Human GeneID(s): 29994

Immunogen

Peptide with sequence C-YFEKKWTDTFKVS, from the C Terminus of the protein sequence according to NP_038478.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:4000.
Western blot: Preliminary experiments gave an approx 35kDa band in Testis and Brain lysates after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 221kDa according to NP_038478. The 35kDa band was successfully blocked by incubation with the immunizing peptide.
IHC: In paraffin embedded Human Small Intestine shows primarily nuclear staining in a number of cells of the lamina propria. Recommended concentration, 1-2µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Cow

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