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Goat Anti-TIF1A / TRIM24 Antibody (EB05807)

Related Products: EBP05807 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB05807 Goat Anti-TIF1A / TRIM24 Antibody Pep-ELISA, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
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EB05807 1
EB05807 (4µg/ml) staining of paraffin embedded Human Breast cancer. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: TRIM24, TIF1, transcriptional intermediary factor 1, PTC6, TF1A, TIF1A, Tif1a, hTIF1, TIF1ALPHA, transcriptional intermediary factor 1 alpha, tripartite motif protein 24, tripartite motif-containing 24, RNF82
Official Symbol: TRIM24
Accession Number(s): NP_056989.2; NP_003843.3
Human GeneID(s): 8805
Important Comments: This antibody is expected to recognise both reported isoforms, as represented by NP_003843.3 and NP_056989.2.

Immunogen

Peptide with sequence C-KRLKSIEERQLLK, from the C Terminus of the protein sequence according to NP_056989.2; NP_003843.3.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Preliminary experiments gave an approx 120kDa band in human kidney and heart lysates at 1ug/ml, however prominent bands were also seen at 25kDa and 45kDa. Please note that currently we cannot find an explanation in the literature for some of the bands we observe given the predicted sizes of approx. 117kDa and 121kDa according to NP_003843 and NP_056989. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
IHC: In paraffin embedded Human Breast cancer shows nuclear staining in the distorted lobules. Recommended concentration, 4-6µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Cow

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