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Goat Anti-PI31 / PSMF1 (Isoform 1) Antibody (EB05845)

Related Products: EBP05845 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB05845 Goat Anti-PI31 / PSMF1 (Isoform 1) Antibody Pep-ELISA, WB, IF Yes 100µg specific antibody in 200µl Human, Mouse, Rat $411.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB05845 1
EB05845 (0.3µg/ml) staining of HEK293 (A), K562 (B) and (1ug/ml) LNCaP (C) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB05845 2
EB05845 (1µg/ml) staining of KNRK (A), NIH3T3 (B and (0.3ug/ml) Neuro2a (C) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB05845 3
EB05845 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing endoplasmic reticulum and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Click here to download PDF datasheet.

Target Protein

Principal Names: PSMF1, PI31, proteasome (prosome, macropain) inhibitor subunit 1 (PI31), RP4-545L17.1, proteasome inhibitor hP131 subunit, proteasome inhibitor subunit 1
Official Symbol: PSMF1
Accession Number(s): NP_006805.2
Human GeneID(s): 9491
Non-Human GeneID(s): 228769 (mouse)
Important Comments: This antibody is expected to recognize isoform 1 (NP_006805.2 and NP_848693.2).

Immunogen

Peptide with sequence C-DHLPPPGYDDMYL, from the C Terminus of the protein sequence according to NP_006805.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 30kDa band observed in lysates of cell line HEK293,, approx 30-32kDa in lysates of cell lines K562, NIH3T3, Neuro2a and KNRK, and approx. 35kDa in lysates of cell line LNCaP (calculated MW of 29.8kDa according to Human NP_006805.2 and Rat NP_001094475.1, and 28.7kDa according to Mouse NP_997611.1). Recommended concentration: 0.3-1µg/ml Primary incubation 1 hour at room temperature.

Immunofluorescence: Strong expression of the protein seen in the endoplasmic reticulum and cytoplasm of A431 cells. Recommended concentration: 10µg/ml.

Flow Cytometry:A customer reported weak staining of A431 cells at 10ug/ml.

Species Reactivity

Tested: Human, Mouse, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow

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