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Goat Anti-RNF13 / RZF (isoform 1 and 3) Antibody (EB05854)

Related Products: EBP05854 - Immunizing Peptide, EBP08552 - Immunizing Peptide, Goat Anti-RNF13 / RZF Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB05854 Goat Anti-RNF13 / RZF (isoform 1 and 3) Antibody Pep-ELISA, WB, IHC, FC Yes 100µg specific antibody in 200µl Human $450.00 Elite_Plus

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ARP American Research Products, Inc

ARP American Research Products, Inc
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Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
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EB05854 1
EB05854 (2µg/ml) staining of A431 (A) and HeLa (B) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB05854 2
EB05854 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
EB05854 3
EB05854 (5µg/ml) staining of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
EB05854 4
EB05854 Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: RNF13, ring finger protein 13, RZF, RING zinc finger protein, MGC13689, FLJ93817
Official Symbol: RNF13
Accession Number(s): NP_009213.1; NP_001365216.1; NP_001365220.1
Human GeneID(s): 11342
Important Comments: Reported variants represent identical protein (NP_009213.1; NP_899237.1).

Immunogen

Peptide with sequence C-PNGERDYNIANTV, from the C Terminus of the protein sequence according to NP_009213.1; NP_001365216.1; NP_001365220.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:1000.
Western blot: Approx. 42kDa band observed in lysates of cell line A431 and in preliminary testing of Human Lung lysate, and approx. 45kDa in lysates of cell line HeLa and in preliminary testing of U251 cell lysate (calculated MW of 42.8kDa according to NP_009213.1). Recommended concentration: 1-3ug/ml. Primary incubation 1 hour at room temperature.
IHC: Paraffin embedded Human Kidney. Recommended concentration: 5µg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

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