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Goat Anti-ALS2CR2 / ILPIP Antibody (EB05865)

Related Products: EBP05865 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB05865 Goat Anti-ALS2CR2 / ILPIP Antibody Pep-ELISA, WB, IHC, IF Yes 100µg specific antibody in 200µl Human $450.00 Elite_Plus

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB05865 1
EB05865 (1µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB05865 2
HEK293 overexpressing ILPIP (RC203432) and probed with EB05865 (mock transfection in first lane), tested by Origene.
EB05865 3
EB05865 (3.8µg/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB05865 4
EB05865 Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Click here to download PDF datasheet.

Target Protein

Principal Names: STRADB, ALS2CR2, ILPIP, PAPK, ILPIPA, CALS-21, PRO1038, amyotrophic lateral sclerosis 2 (juvenile) chromosome region, candidate 2, ILP-interacting protein ILPIPA, STE20-related kinase adaptor beta, STRAD beta, pseudokinase ALS2CR2
Official Symbol: STRADB
Accession Number(s): NP_061041.2; AAF71042.1
Human GeneID(s): 55437
Important Comments: This is expected to recognise both human isoforms: ILPIP-alpha (NP_061041.2) and ILPIP-beta (AAF71042.1).

Immunogen

Peptide with sequence CDFPDEKDSYWEF, from the C Terminus of the protein sequence according to NP_061041.2; AAF71042.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 35kDa band observed in Human Heart, Liver and Muscle lysates (calculated MW of 47.0kDa according to NP_061041.2 (alpha isoform) and 31.2kDa according to AAF71042.1 (beta isoform). In transfected HEK293 transiently expressing ILPIP a band of approx. 48kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 1-3µg/ml.
IHC: In paraffin embedded Human Heart shows staining of myocardial fibres in transverse section. Recommended concentration: 3-5µg/ml.

Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of U251 cells. Recommended concentration: 10µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Dog, Cow

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